During development of the limb musculature there is an eclipse of the myoblasts from the time first formed until the limb musculature begins to develop. The formation of the muscles of the limb is from cells of somite origin that migrate into the forming limb bud. While it is possible to identify myogenic cells in the somite prior to when migration occurs, and to determine the time frame during which migration occurs, there is a hiatus of about 34 hours during which it is difficult to identify or culture myogenic cells from the limb. In part this is because it is difficult to distinguish myogenic cells that migrate from those that do not. There are no markers that distinguish the myogenic cells of the somite that form the axial muscles and therefore stay in place, and from the myogenic cells that migrate to form the peripheral muscles of the body. Thus it unclear if the cell commitment to different myogenic fates occurs during somite formation, or during their sojourn to the limb bud.
View details for Web of Science ID A1992BX82U00001
View details for PubMedID 1341029