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GENERATION OF A HUMAN HYBRIDOMA PRODUCING A PURE ANTI-HLA-A2 MONOCLONAL-ANTIBODY HUMAN IMMUNOLOGY WALLACE, E. F., Foung, S. K., Bradbury, K., PASK, S. L., GRUMET, F. C. 1990; 28 (1): 65-69

Abstract

We report the production and characterization of a human monoclonal IgM hybridoma antibody recognizing antigen HLA-A2. B lymphocytes obtained from the peripheral blood of a multiparous volunteer 1 week postpartum were transformed in vitro by Epstein-Barr virus, screened by a microlymphocytotoxicity assay, and electrofused with the heterohybridoma fusion partner, K6H6/B5. A specifically anti-A2 secreting hybridoma cell line. MBW1, was then identified and cloned. The cytotoxic IgM antibody produced showed complete correlation (r = 1.00) with the A2 antigen on a large panel of unrelated donors' lymphocytes, and no cross-reactivity with A28, Aw68, or Aw69 antigens was observed.

View details for Web of Science ID A1990CZ82300007

View details for PubMedID 2160447