STORAGE AND TRANSPORT OF CULTURES FOR HERPES-SIMPLEX VIRUS, TYPE-2 AMERICAN JOURNAL OF CLINICAL PATHOLOGY YEAGER, A. S., MORRIS, J. E., Prober, C. G. 1979; 72 (6): 977-979

Abstract

Tryptic soy broth and brain-heart infusion broth maintained herpes simplex virus within +/- 1 serial dilution of the original titer in all of 51 samples held for one to three days at 4 C. In contrast, holding temperatures of -20 C or 22 C resulted in losses of titer of 10(2) or more. Holding periods as long as five days prior to inoculation did not delay the appearance of the cytopathic effect following inoculation. Unmodified tryptic soy broth or brain-heart infusion broth and holding periods as long as three days at 4 C prior to arrival in the laboratory give as satisfactory results as do traditional viral culture media and prompt inoculation of the specimen.

View details for Web of Science ID A1979HZ87000016

View details for PubMedID 229726