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CHARACTERIZATION OF LIPID-COMPOSITION IN STIMULATED HUMAN-LYMPHOCYTES BY H-1-NMR
CHARACTERIZATION OF LIPID-COMPOSITION IN STIMULATED HUMAN-LYMPHOCYTES BY H-1-NMR BIOCHIMICA ET BIOPHYSICA ACTA Sze, D. Y., Jardetzky, O. 1990; 1054 (2): 198-206Abstract
Recent in vivo NMR studies have raised interest in the structural changes of cellular lipids during proliferative activity. We investigated the changes in plasma membrane lipid and total cell lipid during mitogenically-stimulated proliferation of human peripheral blood lymphocytes by extraction of lipids and assay by 500 MHz 1H-NMR. Resonances were assigned using one- and two-dimensional spectroscopic techniques, and signals unique to certain species of lipid were identified. Choline and ethanolamine-containing lipids, glycerophospholipid backbones, sphingolipids, cholesterol, plasmalogens and triacylglycerols were readily detected. Resolution of a number of lipid species was not possible, despite the use of high-resolution techniques. NMR values for proliferation-induced changes in the most easily determined parameters, namely the total cholesterol to total phospholipid molar ratio, and phosphatidylcholine, phosphatidylethanolamine and sphingolipid composition, were found to agree with traditional methods. Differences in phospholipid and fatty acid profiles were found between plasma membranes and total cell lipid for resting values and for response to mitogen.
View details for Web of Science ID A1990DZ01600008
View details for PubMedID 2400782