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ENZYMATIC DEGLYCOSYLATION OF THYROID-STIMULATING HORMONE WITH PEPTIDE N-GLYCOSIDASE-F AND "ENDO-BETA-N-ACETYLGLUCOSAMINIDASE-F BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Lee, K. O., Gesundheit, N., Chen, H. C., Weintraub, B. D. 1986; 138 (1): 230-237

Abstract

We investigated the ability of two enzymes, peptide N-glycosidase F (PNGase F) and endo-beta-N-acetylglucosaminidase F (Endo F), to deglycosylate microgram quantities of bovine TSH and its subunits under nondenaturing conditions. One oligosaccharide chain could be selectively removed from the alpha subunit by PNGase F, and all the oligosaccharide chains from both subunits could be removed by Endo F. These methods of enzymatic deglycosylation should permit study of the functional role of each N-linked carbohydrate chain of various glycoprotein hormones.

View details for Web of Science ID A1986D241700033

View details for PubMedID 3091014