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MANNOSE-SENSITIVE AND GAL-GAL BINDING ESCHERICHIA-COLI PILI FROM RECOMBINANT STRAINS - CHEMICAL, FUNCTIONAL, AND SEROLOGICAL PROPERTIES JOURNAL OF EXPERIMENTAL MEDICINE OHANLEY, P., LARK, D., Normark, S., FALKOW, S., SCHOOLNIK, G. K. 1983; 158 (5): 1713-1719

Abstract

Chromosomal genes encoding the MS and Gal-Gal binding properties have been cloned into separate recombinants and their respective pili characterized. Hapten inhibition of hemagglutination with synthetic carbohydrate receptor analogues and carbohydrate-adsorbed latex agglutination studies indicate that Gal-Gal and MS pili collectively exhibit the binding properties of the parent strain. MS pili migrated in SDS-PAGE with an Mr of 19 kdaltons and 17 kdaltons; the Mr of Gal-Gal pili was 17.5 kdaltons. The pili are chemically similar by amino acid composition and when the N-terminal cysteines are aligned, 8 of the 13 residues between positions 9 and 22 are homologous. Further, carboxy-terminal sequence homology was inferred from the carboxypeptidase digestion of a MS pili and the sequence of a carboxy-terminal tryptic peptide from Gal-Gal pili.

View details for Web of Science ID A1983RQ28400025

View details for PubMedID 6195290