Notice: Users may be experiencing issues with displaying some pages on stanfordhealthcare.org. We are working closely with our technical teams to resolve the issue as quickly as possible. Thank you for your patience.
 

Learn about the flu shot, COVID-19 vaccine, and our masking policy »

Stanford Health Care

RECOGNITION OF HLA-A2 BY CYTO-TOXIC LYMPHOCYTES-T AFTER DNA TRANSFER INTO HUMAN AND MURINE CELLS SCIENCE VANDERIJN, M., Bernabeu, C., ROYERPOKORA, B., Weiss, J., Seidman, J. G., Devries, J., Spits, H., TERHORST, C. 1984; 226 (4678): 1083-1085

Abstract

A gene coding for the major histocompatibility antigen HLA-A2 was transferred into human HLA-A2 negative M1 cells and murine L cells. Following transfection, these cells expressed molecules at the cell surface that are biochemically indistinguishable from HLA-A2 antigens on the human cell line JY from which the HLA-A2 gene was isolated. The M1A2 cells were recognized and lysed by a cytolytic T-cell clone specific for HLA-A2. The transfected L cells which express HLA-A2 in association with human beta 2-microglobulin were not lysed by this T-cell clone. The specific cytolysis of M1A2 cells could be inhibited by monoclonal antibodies to HLA-A2, and monoclonal antibodies to T3, T8, and LFA-1 on cytotoxic T lymphocytes. These results suggest that killing by allospecific T cells requires HLA-A2 antigens as well as other species-specific structures on the target cell surface.

View details for Web of Science ID A1984TT08100028

View details for PubMedID 6333726