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NONISOTOPIC HYBRIDIZATION ASSAY FOR DETERMINATION OF RELATIVE AMOUNTS OF GENOTYPIC HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ZIDOVUDINE RESISTANCE JOURNAL OF CLINICAL MICROBIOLOGY Eastman, P. S., Boyer, E., Mole, L., Kolberg, J., Urdea, M., Holodniy, M. 1995; 33 (10): 2777-2780

Abstract

A nonisotopic hybridization assay for human immunodeficiency virus genotypic zidovudine resistance determination is described. Biotinylated PCR product was hybridized with enzyme-labeled probes for wild-type or resistant mutant sequences and detected colorimetrically or chemiluminescently in a microplate format. Changes in mutant-to-wild-type ratios allow for the monitoring of longitudinal patient samples.

View details for Web of Science ID A1995RV56500050

View details for PubMedID 8567926