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Interlaboratory comparison of sequence-specific PCR and ligase detection reaction to detect a human immunodeficiency virus type 1 drug resistance mutation JOURNAL OF CLINICAL MICROBIOLOGY Shafer, R. W., Winters, M. A., MAYERS, D. L., JAPOUR, A. J., Kuritzkes, D. R., Weislow, O. S., White, F., ERICE, A., SANNERUD, K. J., Iversen, A., Pena, F., Dimitrov, D., Frenkel, L. M., REICHELDERFER, P. S. 1996; 34 (7): 1849-1853

Abstract

Sequence-specific PCR was used in six laboratories and a ligase detection reaction was used in one laboratory to detect the zidovudine-resistance mutation at codon 215 of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase DNA. The genotypes of 27 different clinical samples, including cultured HIV-1 isolates, peripheral blood mononuclear cells, and plasma, were correctly identified by 140 of 154 (91%) assays. The sensitivity for detecting a mutation was 96% for HIV-1 reverse transcriptase DNA clone mixtures containing 30% mutant DNA and 62% for mixtures containing 6% mutant DNA.

View details for Web of Science ID A1996UR41600058

View details for PubMedID 8784610

View details for PubMedCentralID PMC229135