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Genomic organization, 5 ' flanking enhancer region, and chromosomal assignment of the cell cycle gene, p55Cdc MOLECULAR GENETICS AND METABOLISM WEINSTEIN, J., Karim, J., Geschwind, D. H., Nelson, S. F., Krumm, J., Sakamoto, K. M. 1998; 64 (1): 52-57

Abstract

p55Cdc is a mammalian homologue of a family of cell cycle proteins from widely divergent species, which contains WD repeats and has been implicated in cell cycle-regulated ubiquitin-mediated proteolysis. p55Cdc is highly expressed in proliferating but not in differentiated or growth-arrested cells. The expression, phosphorylation, and degradation of this protein have been shown to be cell cycle-regulated. We analyzed a 5.3-kb genomic region that contains the entire rat p55Cdc gene. The gene contains 10 exons ranging in size from 97 to 373 bp. The promoter region has a TAT box, four E-box consensus sequences, and potential binding sites for cell cycle-specific transcription factors. In transient transfection assays, a construct containing a 1000-nucleotide p55Cdc promoter region upstream of the chloramphenicol acetyltransferase reporter gene demonstrated a 12-fold increase in transcriptional activity. Finally, using radiation hybrid mapping techniques, we localized this gene to the human chromosome, 9q13-21.

View details for Web of Science ID 000074925700008

View details for PubMedID 9682218