Two-photon optogenetics of dendritic spines and neural circuits NATURE METHODS Packer, A. M., Peterka, D. S., Hirtz, J. J., Prakash, R., Deisseroth, K., Yuste, R. 2012; 9 (12): 1202-U103

Abstract

We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We applied the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual spines. Using a spatial light modulator, we split the laser beam onto several neurons and performed simultaneous optogenetic activation of selected neurons in three dimensions.

View details for DOI 10.1038/NMETH.2249

View details for Web of Science ID 000312093500025

View details for PubMedID 23142873

View details for PubMedCentralID PMC3518588