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Abstract
We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We applied the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual spines. Using a spatial light modulator, we split the laser beam onto several neurons and performed simultaneous optogenetic activation of selected neurons in three dimensions.
View details for DOI 10.1038/NMETH.2249
View details for Web of Science ID 000312093500025
View details for PubMedID 23142873
View details for PubMedCentralID PMC3518588