Enabling Autologous Human Liver Regeneration With Differentiated Adipocyte Stem Cells CELL TRANSPLANTATION Xu, D., Nishimura, T., Zheng, M., Wu, M., Su, H., Sato, N., Lee, G., Michie, S., Glenn, J., Peltz, G. 2014; 23 (12): 1573-1584

Abstract

We developed a novel method for differentiating adipocyte-derived stem cells (ASCs) into hepatocyte-like cells (iHeps). ASCs are cultured as spherical cellular aggregates, and are then induced by culture in chemically defined media for a short time period to differentiate into spherical-culture iHeps (SCi-Heps). SCi-Heps have many of the in vitro functional properties of mature hepatocytes, and they can stably reconstitute functioning human liver in vivo in a murine model system, and implantation studies demonstrate that SCi-Heps have a very low malignant potential. All human liver regenerative procedures, including ultrasound-guided direct liver implantation, are scalable and appropriate for human clinical use. These methods can be used to achieve the major promise of regenerative medicine; it may now be possible to regenerate human liver using autologous stem cells obtained from a readily accessible tissue.

View details for DOI 10.3727/096368913X673432

View details for Web of Science ID 000346626300009

View details for PubMedID 24148223