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Single-cell enumeration of an uncultivated TM7 subgroup in the human subgingival crevice APPLIED AND ENVIRONMENTAL MICROBIOLOGY Ouverney, C. C., Armitage, G. C., Relman, D. A. 2003; 69 (10): 6294-6298

Abstract

Specific oligonucleotide hybridization conditions were established for single-cell enumeration of uncultivated TM7 and IO25 bacteria by using clones expressing heterologous 16S rRNA. In situ analysis of human subgingival crevice specimens revealed that a greater proportion of samples from sites of chronic periodontitis than from healthy sites contained TM7 subgroup IO25. In addition, IO25 bacterial cells from periodontitis site samples were more abundant and fourfold longer than IO25 cells from healthy site samples.

View details for DOI 10.1128/AEM.69.10.6294-6298.2003

View details for Web of Science ID 000185881300072

View details for PubMedID 14532094