Effects of GP2 expression on secretion and endocytosis in pancreatic AR4-2J cells BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Yu, S., Hao, Y., Lowe, A. W. 2004; 322 (1): 320-325

Abstract

GP2 is the major membrane protein present in secretory granules of the exocrine pancreas. GP2's function is unknown, but a role in digestive enzyme packaging or secretion from secretory granules has been proposed. In addition, GP2 has been proposed to influence endocytosis and membrane recycling following stimulated secretion. Adenovirus-mediated GP2 overexpression in the rat pancreatic cell line AR4-2J was used to study its impact on digestive enzyme secretion and membrane recycling. Immunoelectron microscopy showed that GP2 and amylase co-localized in secretory granules in infected AR4-2J cells. CCK-8 stimulation resulted in a fourfold increase in amylase secretion with or without GP2 expression. GP2 expression also did not influence endocytosis following CCK-8 stimulation. Thus, GP2 expression in AR4-2J cells does not affect amylase packaging in secretory granules or stimulated secretion. GP2 expression also does not influence membrane recycling in response to stimulated stimulation in AR4-2J cells.

View details for DOI 10.1016/j.bbrc.2004.07.120

View details for Web of Science ID 000223581000045

View details for PubMedID 15313209