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DETECTION OF T-CELL AND B-CELL ANTIGENS WITH HYBRIDOMA MONOCLONAL ANTIBODIES - A BIOTIN-AVIDIN-HORSERADISH PEROXIDASE METHOD JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY Warnke, R., Levy, R. 1980; 28 (8): 771-776

Abstract

Acetone-fixed frozen sections of reactive lymph node, spleen, three B cell lymphomas, and three T cell lymphomas were studied for the presence of Ia-like antigens and two T cell antigens. Detection of the binding of the hybridoma monoclonal antibodies to these antigens took advantage of the biotin-avidin interaction. The detection method employed a biotin conjugate of goat anti-mouse antibody and an avidin conjugate of horseradish peroxidase. B cell lymphoma cells stained for Ia. The cell lymphomas were shown to be heterogeneous for the expression of the two T cell antigens. these three antigens were generally not detectable after 10% formalin fixation of B-5 fixation. Detection of these antigens and the method employed should prove useful in the immunologic categorization of human lymphomas.

View details for Web of Science ID A1980KV02100004

View details for PubMedID 7003003