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SUPPRESSION OF ENDOGENOUS AVIDIN-BINDING ACTIVITY IN TISSUES AND ITS RELEVANCE TO BIOTIN-AVIDIN DETECTION SYSTEMS JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY Wood, G. S., Warnke, R. 1981; 29 (10): 1196-1204

Abstract

As biotin-avidin systems continue to be developed for applications involving single cells, cell suspensions, and especially tissue sections, the need arises for a method of blocking endogenous avidin-binding activity. One such method is described and its proposed mechanism is discussed. Utilizing this method, endogenous avidin-binding activity was detected and suppressed in selected human and murine tissues, thus facilitating the interpretation of specific immunohistochemical staining utilizing hybridoma monoclonal antibodies in a biotin-avidin-horseradish peroxidase detection system.

View details for Web of Science ID A1981MG07500011

View details for PubMedID 7028859