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CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa: A Brief Methodology. Methods in molecular biology (Clifton, N.J.) Wu, W. H., Tsai, Y. T., Justus, S. n., Cho, G. Y., Sengillo, J. D., Xu, Y. n., Cabral, T. n., Lin, C. S., Bassuk, A. G., Mahajan, V. B., Tsang, S. H. 2018; 1715: 191–205

Abstract

CRISPR/Cas9 genome engineering is currently the leading genome surgery technology in most genetics laboratories. Combined with other complementary techniques, it serves as a powerful tool for uncovering genotype-phenotype correlations. Here, we describe a simplified protocol that was used in our publication, CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa, providing an overview of each section of the experimental process.

View details for PubMedID 29188514