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Evaluating human T cell receptor gene expression by PCR. Current protocols in immunology / edited by John E. Coligan ... [et al.] Gussoni, E., Panzara, M. A., Steinman, L. 2001; Chapter 10: Unit 10 26-?

Abstract

This unit describes the use of PCR to characterize and quantify rearranged transcripts from specific T cell receptor variable gene families in human tissue and peripheral blood lymphocytes. The strategy outlined in this unit has been extensively used on different sources of human tissue including brain, spinal cord, and skeletal muscle. A protocol is provided to clone and sequence PCR-amplified cDNA transcripts to study the junctional diversity of the expressed genes. A support protocol describes a method for reverse transcribing total RNA to make the cDNA required by the other protocols.

View details for DOI 10.1002/0471142735.im1026s22

View details for PubMedID 18432691