Acyloxyacyl hydrolase modulates depressive-like behaviors through aryl hydrocarbon receptor AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY Aguiniga, L. M., Yang, W., Yaggie, R. E., Schaeffer, A. J., Klumpp, D. J., Clemens, J., Hanno, P., Kirkali, Z., Kusek, J. W., Landis, J., Lucia, M., Moldwin, R. M., Mullins, C., Pontari, M. A., van Bokhoven, A., Osypuk, A. A., Dayton, R., Triolo, C. S., Jonscher, K. R., Sullivan, H. T., Wilson, R., Grasmick, Z. D., Bavendam, T. G., Barrell, T., Doe, R., Farrar, J. T., Fernando, M., Gallagher, L., Hou, X., Howard, T., Jemielita, T., Kuzla, N., Newcomb, C., Robinson-Garvin, N., Smith, S., Stephens-Shields, A., Wang, Y., Wang, X., (Vania) Apkarian, A., Arroyo, C., Bass, M., Cella, D., Farmer, M. A., Fitzgerald, C., Gershon, R., Griffith, J. W., Heckman, C. J., Jiang, M., Keefer, L., Lloyd, R., Marko, D. S., Michniewicz, J., Miller, R., Parrish, T., Tu, F., Yaggi, R., Mayer, E. A., Rodriguez, L., Alger, J., Ashe-McNalley, C. P., Ellingson, B., Heendeniya, N., Kilpatrick, L., Cara, K., Kutch, J., Labus, J. S., Naliboff, B. D., Randal, F., Smith, S. R., Kreder, K. J., Bradley, C. S., Eno, M., Greiner, K., Luo, Y., Lutgendorf, S. K., O'Donnell, M. A., Ziegler, B., Schrepf, A., Hardy, I., Magnotta, V., Erickson, B., Clauw, D. J., As-Sanie, S., Berry, S., Grayhack, C., Halvorson, M. E., Harris, R., Harte, S., Ichesco, E., Oldendorf, A., Scott, K. A., Williams, D. A., Buchwald, D., Afari, N., Bacus, T., Edwards, T., Krieger, J., Maravilla, K., Miller, J., Patrick, D., Qin, X., Richey, S., Risques, R., Robertson, K., Ross, S. O., Spiro, R., Strachan, E., Sundsvold, T. J., Sutherland, S., Yang, C. C., Andriole, G. L., Lai, H., Bristol, R. L., Gereau, R. W., Hong, B. A., Klim, A. P., Sutcliffe, S., Vetter, J., Song, D. G., Milbrandt, M., Haroutounian, S., Vijairania, P., Parker (Chaturvedi), K., Hung, T., Colditz, G., Gardner, V. C., Henderson, J. P., Spitznagle, T. M., Pakpahan, R., James, A., Yan, Y., Langston, M., Hong, B., Mueller, S., Crowley, J., Vogt, S., Hultgren, S., Nguyen, N., Blasche, G., Qiu, C., Cupps, L., Bok, S., Hooten, T. M., Grullon, L., Atis, N., Ness, T. J., Deutsch, G., Den Hollander, J., Corbitt, B. D., Bradley, L., North, C. S., Downs, D., Anger, J., Ackerman, J., Ackerman, A., Cha, J., Eilber, K., Freeman, M., Funari, V., Kim, J., Van Eyk, J., Yang, W., Moses, M. A., Briscoe, A. C., Briscoe, D., Curatolo, A., Froehlich, J., Lee, R. S., Sachdev, M., Solomon, K. R., Steen, H., Mackey, S., Bagarinao, E., Foster, L. C., Hubbard, E., Johnson, K. A., Martucci, K. T., McCue, R. L., Moericke, R. R., Nilakantan, A., Noor, N., Nickel, J., Ehrlich, G. D., MAPP Res Network Study Grp 2019; 317 (2): R289–R300

Abstract

Corticotropin-releasing factor (CRF) regulates stress responses, and aberrant CRF signals are associated with depressive disorders. Crf expression is responsive to arachidonic acid (AA), where CRF is released from the hypothalamic paraventricular nucleus (PVN) to initiate the hypothalamic-pituitary-adrenal axis, culminating in glucocorticoid stress hormone release. Despite this biological and clinical significance, Crf regulation is unclear. Here, we report that acyloxyacyl hydrolase, encoded by Aoah, is expressed in the PVN, and Aoah regulates Crf through the aryl hydrocarbon receptor (AhR). We previously showed that AOAH-deficient mice mimicked interstitial cystitis/bladder pain syndrome, a condition frequently associated with comorbid anxiety and depression. With the use of novelty-suppressed feeding and sucrose preference assays to quantify rodent correlates of anxiety/depression, AOAH-deficient mice exhibited depressive behaviors. AOAH-deficient mice also had increased CNS AA, increased Crf expression in the PVN, and elevated serum corticosterone, consistent with dysfunction of the hypothalamic-pituitary-adrenal axis. The human Crf promoter has putative binding sites for AhR and peroxisome proliferator-activated receptor (PPAR?). PPAR? did not affect AA-dependent Crf expression in vitro, and conditional Ppar? knockout did not alter the AOAH-deficient depressive phenotype, despite previous studies implicating PPAR? as a therapeutic target for depression. In contrast, Crf induction was mediated by AhR binding sites in vitro and increased by AhR overexpression. Furthermore, conditional Ahr knockout rescued the depressive phenotype of AOAH-deficient mice. Finally, an AhR antagonist rescued the AOAH-deficient depressive phenotype. Together, our results demonstrate that Aoah is a novel genetic regulator of Crf mediated through AhR, and AhR is a therapeutic target for depression.

View details for DOI 10.1152/ajpregu.00029.2019

View details for Web of Science ID 000481616900008

View details for PubMedID 31017816