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The Paracrine Function of Mesenchymal Stem Cells in Response to Pulsed Focused Ultrasound

The Paracrine Function of Mesenchymal Stem Cells in Response to Pulsed Focused Ultrasound CELL TRANSPLANTATION Razavi, M., Rezaee, M., Telichko, A., Inan, H., Dahl, J., Demirci, U., Thakor, A. S. 2020; 29: 963689720965478

Abstract

We studied the paracrine function of mesenchymal stem cells (MSCs) derived from various sources in response to pulsed focused ultrasound (pFUS). Human adipose tissue (AD), bone marrow (BM), and umbilical cord (UC) derived MSCs were exposed to pFUS at two intensities: 0.45 W/cm2 ISATA (310 kPa PNP) and 1.3 W/cm2 ISATA (540 kPa PNP). Following pFUS, the viability and proliferation of MSCs were assessed using a hemocytometer and confocal microscopy, and their secreted cytokine profile determined using a multiplex ELISA. Our findings showed that pFUS can stimulate the production of immunomodulatory, anti-inflammatory, and angiogenic cytokines from MSCs which was dependent on both the source of MSC being studied and the acoustic intensity employed. These important findings set the foundation for additional mechanistic and validation studies using this novel noninvasive and clinically translatable technology for modulating MSC biology.

View details for DOI 10.1177/0963689720965478

View details for Web of Science ID 000606584100044

View details for PubMedID 33028105

View details for PubMedCentralID PMC7784560