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Selective femtosecond laser ablation via two-photon fluorescence imaging through a multimode fiber

Selective femtosecond laser ablation via two-photon fluorescence imaging through a multimode fiber BIOMEDICAL OPTICS EXPRESS Kakkava, E., Romito, M., Conkey, D. B., Loterie, D., Stankovic, K. M., Moser, C., Psaltis, D. 2019; 10 (2): 423-433

Abstract

We demonstrate the ability of a multimode fiber probe to provide two-photon fluorescence (TPF) imaging feedback that guides the femtosecond laser ablation (FLA) in biological samples for highly selective modifications. We implement the system through the propagation of high power femtosecond pulses through a graded-index (GRIN) multimode fiber and we investigate the limitations posed by the high laser peak intensities required for laser ablation. We demonstrate that the GRIN fiber probe can deliver laser intensities up to 1.5x1013 W/cm2, sufficient for the ablation of a wide range of materials, including biological samples. Wavefront shaping through an ultrathin probe of around 400 µm in diameter is used for diffraction limited focusing and digital scanning of the focus spot. Selective FLA of cochlear hair cells is performed based on the TPF images obtained through the same multimode fiber probe.

View details for DOI 10.1364/BOE.10.000423

View details for Web of Science ID 000457500300004

View details for PubMedID 30800490

View details for PubMedCentralID PMC6377891