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Gradient Multinozzle 3D Printing.

Gradient Multinozzle 3D Printing. bioRxiv : the preprint server for biology Rosalia, L., Sinha, S., Weiss, J. D., Hsia, S., Solberg, F. S., Sharir, A., Shibata, M., Du, J., Mosle, K., Rutsche, D., Rao, Z. C., Tam, T., Rankin, T., Wang, Q., Williams, C. M., Klich, J. H., Reed, A. K., Appel, E. A., Ma, M., Skylar-Scott, M. A. 2026

Abstract

Direct ink writing is compatible with an expansive materials palette. While enabling diverse applications, this materials versatility brings significant bottlenecks in ink formulation, often requiring the mixing, printing, and testing of dozens to hundreds of ink compositions over the course of a project. To accelerate ink-space exploration, we introduce gradient embedded multinozzle (GEM) printheads that combine the high-throughput parallelized printing of multinozzles with combinatorial ink mixing. These printheads allow simultaneous mixing of two-, three-, and four-input inks which are distributed to printer nozzles to create complex 3D structures with graded compositions of inks. Using a two-way GEM printhead, we validate cell compatibility by printing scaffolds containing various concentrations of fibroblasts and observing non-linear compaction behaviours. We next test a three-way GEM multinozzle to print ten compositions of di- and multi-functionalized poly(ethylene-glycol) diacrylate hydrogel tri-leaflet valves, optimizing for stiffness, swelling ratio, and toughness. Our GEM multinozzles are compatible with open-source printers and either pressure- or volume-driven extrusion systems and promise to accelerate iterative ink design and testing.

View details for DOI 10.64898/2026.03.21.712762

View details for PubMedID 41928986