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Abstract
Recent advances in single-cell manipulation technology, whole genome amplification and high-throughput sequencing have now made it possible to sequence the genome of an individual cell. The bioinformatic analysis of these genomes, however, is far more complicated than the analysis of those generated using traditional, culture-based methods. In order to simplify this analysis, we have developed SmashCell (Simple Metagenomics Analysis SHell-for sequences from single Cells). It is designed to automate the main steps in microbial genome analysis-assembly, gene prediction, functional annotation-in a way that allows parameter and algorithm exploration at each step in the process. It also manages the data created by these analyses and provides visualization methods for rapid analysis of the results.The SmashCell source code and a comprehensive manual are available at http://asiago.stanford.edu/SmashCelleoghanh@stanford.eduSupplementary data are available at Bioinformatics online.
View details for DOI 10.1093/bioinformatics/btq564
View details for Web of Science ID 000284430900009
View details for PubMedID 20966005
View details for PubMedCentralID PMC2982155