Skip to main content
 
Notice: Users may be experiencing issues with displaying some pages on stanfordhealthcare.org. We are working closely with our technical teams to resolve the issue as quickly as possible. Thank you for your patience.
 

See our updated masking policy »

Stanford Health Care

"Human, mouse and dog bone marrow show similar mesenchymal stromal cells within a distinctive microenvironment". Experimental hematology Meza-Leon, B., Gratzinger, D., Aguilar-Navarro, A. G., Juarez-Aguilar, F. G., Rebel, V. I., Torlakovic, E., Purton, L. E., Dorantes-Acosta, E. M., Escobar-Sanchez, A., Dick, J. E., Flores-Figueroa, E. 2021

Abstract

Bone marrow stromal cells (BMSCs) are a key part of the hematopoietic niche. Mouse and human BMSCs are recognized by different markers (LepR and NGFR/CD271, respectively). However, there has not been a detailed in situ comparison of both populations within the hematopoietic microenvironment. Moreover, dog BMSCs have not been characterized in situ by any of those makers. We conducted a systematic histopathological comparison of mouse, human and dog BMSCs within their bone marrow architecture and microenvironment. Human and dog CD271+ BMSCs showed similar morphology, frequency, and distribution within trabecular bone marrow to mouse LepR+ BMSCs. However, mouse bone marrow showed higher cellularity and megakaryocyte content. In conclusion, highly comparable bone marrow mesenchymal stromal cells distribution among the three species establishes the validity of using mouse and dog as a surrogate experimental model of hematopoietic stem cell (HSC):BMSCs interactions. However, the distinct differences in adipocyte and megakaryocyte microenvironment content of mouse bone marrow and how they might influence HSC interactions as compared to humans require further study.

View details for DOI 10.1016/j.exphem.2021.06.006

View details for PubMedID 34228982