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Transient and stable transfection in the protozoan parasite Entamoeba invadens MOLECULAR AND BIOCHEMICAL PARASITOLOGY Ehrenkaufer, G. M., Singh, U. 2012; 184 (1): 59-62

Abstract

Entamoeba histolytica is an important human pathogen and a major health problem worldwide. Many aspects of parasite biology can be studied with the exception of stage conversion, which cannot be reproduced adequately in E. histolytica. The reptile parasite Entamoeba invadens is a vital model system for studying stage conversion since it can be induced to undergo both encystation and excystation with high efficiency in vitro. However, functional studies using E. invadens have been limited by the lack of genetic tools in this species. Here, we report a new method for both transient and stable transfection of E. invadens. These new tools will greatly enhance research into Entamoeba development.

View details for DOI 10.1016/j.molbiopara.2012.04.007

View details for Web of Science ID 000305112200011

View details for PubMedID 22561071

View details for PubMedCentralID PMC3358517