Skip to main content
 
Notice: Users may be experiencing issues with displaying some pages on stanfordhealthcare.org. We are working closely with our technical teams to resolve the issue as quickly as possible. Thank you for your patience.
Stanford Health Care

SODIUM PROTON ANTIPORT IS REQUIRED FOR GROWTH OF ESCHERICHIA-COLI AT ALKALINE PH

SODIUM PROTON ANTIPORT IS REQUIRED FOR GROWTH OF ESCHERICHIA-COLI AT ALKALINE PH BIOCHIMICA ET BIOPHYSICA ACTA McMorrow, I., Shuman, H. A., Sze, D., Wilson, D. M., Wilson, T. H. 1989; 981 (1): 21-26

Abstract

Evidence is presented indicating that Escherichia coli requires the Na+/H+ antiporter and external sodium (or lithium) ion to grow at high pH. Cells were grown in plastic tubes containing medium with a very low Na+ content (5-15 microM). Normal cells grew at pH 7 or 8 with or without added Na+, but at pH 8.5 external Na was required for growth. A mutant with low antiporter activity failed to grow at pH 8.5 with or without Na+. On the other hand, another mutant with elevated antiporter activity grew at a higher pH than normal (pH 9) in the presence of added Na+ or Li+. Amiloride, an inhibitor of the antiporter, prevented cells from growing at pH 8.5 (plus Na+), although it had no effect on growth in media of lower pH values.

View details for Web of Science ID A1989AA50300004

View details for PubMedID 2541789