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QUANTITATION AND ESTIMATION OF LYMPHOCYTE SUBSETS IN TISSUE-SECTIONS - COMPARISON WITH FLOW-CYTOMETRY AMERICAN JOURNAL OF CLINICAL PATHOLOGY Garcia, C. F., Weiss, L. M., Lowder, J., KOMOROSKE, C., Link, M. P., Levy, R., Warnke, R. A. 1987; 87 (4): 470-477

Abstract

A quantitation method for lymphocyte subsets in immunoperoxidase-stained frozen tissue sections was compared with flow cytometry in 23 cases of non-Hodgkin's lymphoma. Close correlations were obtained, demonstrating the accuracy of the technic. Weak intensity of fluorescence and fragility of the tumor cells during the fluorescence-activated cell sorter (FACS) analyses were the most likely explanations for a number of the discrepancies observed. The tissue quantitation method was precise, particularly at low values, where it was better than the FACS. A simpler and faster estimation method employing categories within 10 percentage units was also tested in this study; this method correlated as well with the FACS as the quantitation method and gave the best interobserver correlations.

View details for Web of Science ID A1987G546900006

View details for PubMedID 3103419