THE TRANSCRIPTION FACTOR, NM23H2, BINDS TO AND ACTIVATES THE TRANSLOCATED C-MYC ALLELE IN BURKITTS-LYMPHOMA JOURNAL OF BIOLOGICAL CHEMISTRY Ji, L., Arcinas, M., Boxer, L. M. 1995; 270 (22): 13392-13398

Abstract

We have identified an in vivo footprint over the PuF site on the translocated c-myc allele in Burkitt's lymphoma cells. The PuF site on the silent normal c-myc allele was unoccupied. We demonstrated by electrophoretic mobility shift assay, electrophoretic mobility shift assay with antibody, UV cross-linking followed by SDS-gel electrophoresis, and Western analysis that Nm23H2 in B cell nuclear extracts bound to the c-myc PuF site. Transfection experiments with c-myc promoter constructs in both DHL-9 and Raji cells revealed that the PuF site functioned as a positive regulatory element in B cells with a drop in activity with mutation of this site. Access to this site is blocked in the normal silent c-myc allele; these data suggest that the Nm23H2 protein is involved in deregulation of the translocated c-myc allele in Burkitt's lymphoma cells.

View details for Web of Science ID A1995RB43900066

View details for PubMedID 7768941