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CYTOPLASMIC GRANULE FORMATION IN MOUSE PANCREATIC ACINAR-CELLS - EVIDENCE FOR FORMATION OF IMMATURE GRANULES (CONDENSING VACUOLES) BY AGGREGATION AND FUSION OF PROGRANULES OF UNIT SIZE, AND FOR REDUCTIONS IN MEMBRANE-SURFACE AREA AND IMMATURE GRANULE VOLUME DURING GRANULE MATURATION CELL AND TISSUE RESEARCH LEW, S., Hammel, I., GALLI, S. J. 1994; 278 (2): 327-336

Abstract

We used a computer-assisted morphometry approach to analyze quantitatively the process of cytoplasmic granule formation in mouse pancreatic acinar cells stimulated with pilocarpine to induce secretion. Our findings suggest that each condensing vacuole/immature granule of pancreatic acinar cells is formed by the progressive aggregation of 106 to 128 "unit progranules" of narrowly fixed volume, define a range of 7.7 to 9.2 for the factor of volume condensation between the largest immature granules and the mature unit granule, and predict that the formation of a single mature unit granule by the aggregation and fusion of unit progranules involves a net reduction of at least 95% in the amount of membrane surface area associated with these structures.

View details for Web of Science ID A1994PM03400016

View details for PubMedID 8001087