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Down-regulation of hormone-sensitive lipase in sterol ester-laden J774.2 macrophages BIOCHEMICAL JOURNAL JEPSON, C. A., Harrison, J. A., Kraemer, F. B., Yeaman, S. J. 1996; 318: 173-177

Abstract

The development of atherosclerotic plaques in arteries is a key step in atherogenesis, with cholesterol ester accumulation in macrophage-derived foam cells being recognized as a major pathogenic event in this process. In this study, the mouse macrophage cell line J774.2 was induced to accumulate intracellular sterol esters by incubation with 25-hydroxycholesterol in the presence of oleic acid. The accumulation of sterol esters in these cells was found to be accompanied by a marked decrease in the activity of the enzyme responsible for their hydrolysis, namely hormone-sensitive lipase (HSL); Western blotting studies revealed a corresponding decrease in the levels of the HSL polypeptide. Similar findings were obtained after incubation with oxidized low-density lipoprotein or very-low-density lipoprotein. These findings suggest that down-regulation of the expression of HSL is important in cholesterol ester accumulation in macrophages.

View details for Web of Science ID A1996VE10100025

View details for PubMedID 8761468

View details for PubMedCentralID PMC1217604