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RT-PCR analysis of MMP-9 expression in human articular cartilage chondrocytes and synovial fluid cells BIOTECHNIC & HISTOCHEMISTRY Tsuchiya, K., Maloney, W. J., Vu, T., Hoffman, A. R., Schurman, D. J., Smith, R. L. 1996; 71 (4): 208-213

Abstract

This report presents a method for analyzing matrix metalloproteinase-9 (92 kD gelatinase) MMP-9 mRNA expression using reverse transcription polymerase chain reaction (RT-PCR). With this method, chondrocytes isolated from small samples of osteoarthritic cartilage showed significantly elevated signal for MMP-9 mRNA compared to normal cartilage. In addition, cells of synovial fluid samples aspirated from osteoarthritic joints also exhibited MMP-9 expression using this technique. RT-PCR proved to be a sensitive method for assessing MMP-9 regulation in osteoarthritic and normal cartilage, and may provide a useful index of arthritic cartilage and synovial fluid cell metabolism when limited tissue is available.

View details for Web of Science ID A1996VE64300007

View details for PubMedID 8874859