PARTIAL-PURIFICATION AND CHARACTERIZATION OF MAMMARY STIMULATING FACTOR, A PROTEIN WHICH PROMOTES PROLIFERATION OF MAMMARY EPITHELIUM BIOCHEMISTRY PTASHNE, K., HSUEH, H. W., Stockdale, F. E. 1979; 18 (16): 3533-3539

Abstract

This report describes the partial purification and characterization of mammary stimulating factor (MSF), a mitogenic peptide isolated from serum which initiates growth in mouse mammary epithelium. By using ion-exchange chromatography, gel filtration, and isoelectric focusing, MSF was purified 250-fold from porcine serum. It is a heat-stable protein of molecular weight 10,100--10,400 with an isoelectric point of 5.5--6.0. MSF initiates DNA synthesis in vitro in mammary epithelium to a greater extent than in mouse mammary tumor cells (CZF), 3T3 cells, or chick embryo cells. Comparison of the biological, physical, and immunological properties of MSF with other established growth-promoting peptides suggests that MSF is a unique serum factor.

View details for Web of Science ID A1979HF72800015

View details for PubMedID 476066