Abstract

Advances in tissue engineering offer potential alternatives to current mandibular reconstructive techniques; however, before clinical translation of this technology, a relevant animal model must be used to validate possible interventions.To establish the critical-sized segmental mandibular defect that does not heal spontaneously in the rat mandible.Prospective study of mandibular defect healing in 29 Sprague-Dawley rats in an animal laboratory.The rats underwent creation of 1 of 4 segmental mandibular defects measuring 0, 1, 3, and 5 mm. All mandibular wounds were internally fixated with 1-mm microplates and screws and allowed to heal for 12 weeks, after which the animals were killed humanely.Analysis with micro-computed tomography of bony union and formation graded on semiquantitative scales.Seven animals were included in each experimental group. No 5-mm segmental defects successfully developed bony union, whereas all 0- and 1-mm defects had continuous bony growth across the original defect on micro-computed tomography. Three of the 3-mm defects had bony continuity, and 3 had no healing of the bony wound. Bone union scores were significantly lower for the 5-mm defects compared with the 0-, 1-, and 3-mm defects (P?

View details for DOI 10.1001/jamaoto.2013.5669

View details for Web of Science ID 000331369700010

View details for PubMedID 24232293