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Improved serotype-specific dengue virus detection in Trinidad and Tobago using a multiplex, real-time RT-PCR. Diagnostic microbiology and infectious disease Waggoner, J. J., Sahadeo, N. S., Brown, A., Mohamed-Hadley, A., Hadley, D., Carrington, L., Carrington, C. V., Pinsky, B. A. 2015; 81 (2): 105-106

Abstract

Dengue virus (DENV) transmission occurs throughout the Caribbean, though laboratory confirmation and epidemiologic surveillance are limited by the availability of serotype-specific molecular diagnostics. In this study, we show that a serotype-specific DENV multiplex, real-time reverse transcriptase-PCR (RT-PCR) detected DENV RNA in significantly more samples (82/182) than a reference hemi-nested RT-PCR (57/182; P=0.01).

View details for DOI 10.1016/j.diagmicrobio.2014.10.001

View details for PubMedID 25533614

View details for PubMedCentralID PMC4297500