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CLONED MOUSE MAST-CELLS AND NORMAL MOUSE PERITONEAL MAST-CELLS - DETERMINATION OF SEROTONIN CONTENT AND ABILITY TO SYNTHESIZE SEROTONIN INVITRO INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY Weitzman, G., GALLI, S. J., Dvorak, A. M., Hammel, I. 1985; 77 (1-2): 189-191

Abstract

We determined the serotonin (5-hydroxytryptamine, 5HT) content of growth factor dependent mouse mast cell lines or clones, and measured their ability to synthesize and store 3H-5HT from exogenous 5-hydroxy-[G-3H]-tryptophan (3H-5HTP) in vitro. Mast cells grown in vitro synthesized 3H-5HT from 3H-5HTP at rates equal to or greater than those of peritoneal mast cells freshly isolated from normal mice. Furthermore, under usual conditions of culture, mast cell lines or clones contained more 5HT than freshly isolated peritoneal mast cells.

View details for Web of Science ID A1985AJS2400038

View details for PubMedID 3874166