Abstract

Data presented in this paper show that the recognition of keyhole limpet hemocyanin by murine T-cell clones is restricted by products of the I region. These data have been obtained by genetic mapping studies as well as by the use of monoclonal Ia-specific antibodies which inhibit the ability of antigen-presenting cells to effectively present antigen to such T- cell clones. Use of heterozygous antigen-presenting cells derived from crosses between B6.C-H-2bm12 and B10.A(4R) mice have allowed us to show that both trans-complementing I-A products are used for restriction of recognition of KLH. These data were confirmed using monoclonal Ia antibodies to inhibition recognition of KLH by the same T -cell clones. Thus, we have shown that there exist hybrid molecules formed by free combinatorial association of products encoded within the I-A subregion which restrict the recognition of soluble antigen. Additionally, we have shown that the molecule formed by complementation between the alpha chain encoded within the I-E region and a beta chain encoded within the A region (Ae) can fuction effectively in presenting KLH to certain murine T -cell clones. These results support the hypothesis that the recognition of individual antigenic epitopes within large multideterminant antigens is under the control of Ir genes.

View details for Web of Science ID A1981MR12800006

View details for PubMedID 6800947