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Direct subcutaneous injection of polyethylene particles over the murine calvaria results in dramatic osteolysis INTERNATIONAL ORTHOPAEDICS Rao, A. J., Zwingenberger, S., Valladares, R., Li, C., Smith, R. L., Goodman, S. B., Nich, C. 2013; 37 (7): 1393-1398

Abstract

PURPOSE: The murine calvarial model has been widely employed for the in vivo study of particle-induced osteolysis, the most frequent cause of aseptic loosening of total joint replacements. Classically, this model uses an open surgical technique in which polyethylene (PE) particles are directly spread over the calvarium for the induction of osteolysis. We evaluated a minimally invasive modification of the calvarial model by using a direct subcutaneous injection of PE particles. METHODS: Polyethylene (PE) particles were injected subcutaneously over the calvaria of C57BL6J ten-week-old mice ("injection" group) or were implanted after surgical exposure of the calvaria ("open" group) (n?=?5/group). For each group, five additional mice received no particles and served as controls. Particle-induced osteolysis was evaluated two weeks after the procedure using high-definition microCT imaging. RESULTS: Polyethylene particle injection over the calvaria resulted in a 40 %?±?1.8 % decrease in the bone volume fraction (BVF), compared to controls. Using the "open surgical technique", the BVF decreased by 16 %?±?3.8 % as compared to controls (p?

View details for DOI 10.1007/s00264-013-1887-4

View details for Web of Science ID 000320660500028