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High-fat diet induces systemic B-cell repertoire changes associated with insulin resistance. Mucosal immunology Pham, T. D., Chng, M. H., Roskin, K. M., Jackson, K. J., Nguyen, K. D., Glanville, J., Lee, J., Engleman, E. G., Boyd, S. D. 2017

Abstract

The development of obesity-associated insulin resistance is associated with B-lymphocyte accumulation in visceral adipose tissue (VAT) and is prevented by B-cell ablation. To characterize potentially pathogenic B-cell repertoires in this disorder, we performed high-throughput immunoglobulin (Ig) sequencing from multiple tissues of mice fed high-fat diet (HFD) and regular diet (RD). HFD significantly changed the biochemical properties of Ig heavy-chain complementarity-determining region-3 (CDRH3) sequences, selecting for IgA antibodies with shorter and more hydrophobic CDRH3 in multiple tissues. A set of convergent antibodies of highly similar sequences found in the VAT of HFD mice but not RD mice showed significant somatic mutation, suggesting a response shared between mice to a common antigen or antigens. These findings indicate that a simple high-fat dietary intervention has a major impact on mouse B-cell repertoires, particularly in adipose tissues.Mucosal Immunology advance online publication, 19 April 2017; doi:10.1038/mi.2017.25.

View details for DOI 10.1038/mi.2017.25

View details for PubMedID 28422186