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Understanding RNA-Chromatin Interactions Using Chromatin Isolation by RNA Purification (ChIRP) POLYCOMB GROUP PROTEINS: METHODS AND PROTOCOLS Chu, C., Chang, H. Y., Lanzuolo, C., Bodega, B. 2016; 1480: 115–23
Abstract
ChIRP is a novel and easy-to-use technique for studying long noncoding RNA (lncRNA)-chromatin interactions. RNA and chromatin are cross-linked in vivo using formaldehyde or glutaraldehyde, and purified using biotinylated antisense oligonucleotides that hybridize to the target RNA. Co-precipitated DNA is then purified and analyzed by quantitative PCR (qPCR) or high-throughput sequencing.
View details for PubMedID 27659979