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Purification of bovine alpha-lactalbumin by immobilized metal ion affinity chromatography PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY Blomkalns, A. L., Gomez, M. R. 1997; 27 (4): 219–26

Abstract

The milk protein alpha-lactalbumin was isolated from bovine whey protein concentrate solution by immobilized metal ion affinity chromatography (IMAC) using Cu(II)-Chelating Sepharose Fast Flow. Stepwise pH (5.5-3.8) changes in sodium acetate buffer were used to elute the protein selectively, at which time it was concentrated and reapplied to an uncharged Chelating Sepharose Fast Flow column to remove the contaminating Cu(II) ions. A purity of 90% and recovery of 80% was achieved. The described method appears to be suitable for isolation of alpha-lactalbumin in a form adequate for milk formula engineering.

View details for DOI 10.1080/10826069708001280

View details for Web of Science ID A1997YK08800002

View details for PubMedID 9413555