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Kinetics of B cell receptor signaling in human B cell subsets mapped by phosphospecific flow cytometry JOURNAL OF IMMUNOLOGY Irish, J. M., Czerwinski, D. K., Nolan, G. P., Levy, R. 2006; 177 (3): 1581-1589

Abstract

Differences in BCR signaling may govern outcomes as diverse as proliferation and cell death. We profiled BCR signaling kinetics in subsets of primary human B cells using flow cytometry. In the predominant population expressing IgM, BCR cross-linking led to a quick burst of Syk, ERK1/2, and p38 signaling. In contrast, IgG B cells sustained higher per-cell ERK1/2 phosphorylation over time. This dichotomy suggested a mechanism for dampening signals transmitted by IgM. Regulatory phosphatase activity in IgM B cells was BCR-mediated and initiated more slowly than kinase activity. This BCR-mediated phosphatase activity was sensitive to inhibition by H(2)O(2) and required to attenuate IgM BCR signaling. These results provide the first kinetic maps of BCR signaling in primary human B cell subsets and enable new studies of signaling in B cell disorders, such as autoimmunity and cancer.

View details for Web of Science ID 000239140300032

View details for PubMedID 16849466