CD9 mRNA and protein expression levels in mouse slow frozen-rapid thawed oocytes were compared with those in fresh oocytes by using comparative quantitative real time reverse transcription-PCR and semiquantitative Western blot, respectively. The expression levels of both CD9 mRNA and protein in the frozen oocytes were significantly lower than those found in the fresh oocytes.
View details for DOI 10.1016/j.fertnstert.2006.11.130
View details for Web of Science ID 000248716000044
View details for PubMedID 17307168