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Guanine nucleotide depletion mediates translocation of nucleolar proteins, including RNA helicase A (DHX-9)

Guanine nucleotide depletion mediates translocation of nucleolar proteins, including RNA helicase A (DHX-9) 12th International Symposium on Purine and Pyrimidine Metabolism Huang, M., Mitchell, B. S. TAYLOR & FRANCIS INC. 2008: 704–11

Abstract

DHX-9, a member of the DEXH family of RNA helicases, unwinds dsRNA/dsDNA by ATP or GTP-dependent hydrolysis. We asked whether DHX-9 played a role in the GTP depletion-induced inhibition of rRNA synthesis and/or nucleolar disruption. MPA, a specific inhibitor of inosine monophosphate dehydrogenase (IMPDH), induced a rapid translocation of DHX-9 from the nucleolus to the nucleus. EGFP-tagged DHX-9 mutated at the GTP binding site also localized to the nucleus. However, knockdown of DHX-9 by siRNA did not inhibit the rRNA synthesis or cause the nucleolar disruption. Thus, DHX-9 translocation found with IMPDH inhibition does not mediate the inhibition of rRNA synthesis.

View details for DOI 10.1080/15257770802145132

View details for Web of Science ID 000257338600029