Abstract

Cytokine profiles of peripheral blood and other bodily fluids provide diagnostic indicators for assessing inflammatory processes. Menstrual effluent may provide a noninvasive source of biological material for monitoring cytokine levels in blood and in endometrial tissues. This pilot study investigated the potential of measuring cytokines in menstrual effluent, and compared the cytokine profiles of menstrual versus peripheral blood.Seven healthy donors (aged =18 and =45 years) collected menstrual effluent on day 2 of menses. Matched peripheral blood samples were collected by venous blood draw on the same day. Levels of 62 cytokines were measured in all samples by 62-plex Luminex assay.Peripheral blood and menstrual effluent cytokine profiles were tenuously correlated (r 2?=?0.26, p??0.05) for 53/62 cytokines in menstrual effluent versus peripheral blood. Levels of TGF-ß (r 2?=?0.87, p?=?0.002) and CCL7 (r 2?=?0.63, p?=?0.033) were significantly positively correlated between matched menstrual and peripheral blood samples.In this group of study participants, the cytokine profile of menstrual effluent was quantitatively distinct from peripheral blood, and also characterized by higher levels of inflammatory signaling. This pattern of comparative menstrual blood cytokine profiles points to a need for further studies to evaluate the relationship between peripheral and menstrual blood cytokines in broader populations including both healthy and diseased states.

View details for DOI 10.1002/hsr2.1038

View details for PubMedID 36620506

View details for PubMedCentralID PMC9813904