Anti-NC16A IgA from linear IgA bullous dermatosis patients induce neutrophil-dependent subepidermal blistering in mice. The Journal of investigative dermatology Jing, K., Jordan, T. J., Li, N., Burette, S., Yang, B., Marinkovich, M. P., Diaz, L. A., Googe, P., Thomas, N. E., Feng, S., Liu, Z. 2023


Linear IgA bullous dermatosis (LABD) is an acquired autoimmune subepidermal blistering skin disease characterized by circulating and tissue-bound IgA autoantibodies that recognize epitopes within the hemidesmosomal protein BP180, including its NC16A domain. Histologically, LABD has long been defined by neutrophil infiltration and dermal-epidermal separation. However, the pathogenic roles of anti-NC16A IgA and neutrophils in LABD, as well as their interactions, have not been thoroughly studied. We show that passive transfer of patient-derived anti-NC16A IgA induce clinical and histologic LABD pathology in humanized NC16A mice that are reconstituted locally or systemically with human neutrophils. The lesional skin of mice exhibits significantly elevated levels of the neutrophil chemoattractants CXCL-1 and CXCL-2. Furthermore, we show significantly increased levels of the neutrophil chemoattractant IL-8 in blister fluids of LABD patients. This study provides direct evidence that anti-NC16A IgA in LABD patients are pathogenic and interact with neutrophils to mediate tissue injury and subepidermal blister formation. This study further corroborates the importance of neutrophil-mediated tissue injury in LABD disease physiology and establishes a clinically relevant in vivo model system that can be used to systematically dissect the immunopathogenesis of LABD.

View details for DOI 10.1016/j.jid.2023.05.027

View details for PubMedID 37437774