Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.
View details for DOI 10.1038/NMETH.1426
View details for Web of Science ID 000275058200018
View details for PubMedID 20139967
View details for PubMedCentralID PMC2892897