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Chemical inactivation strategies for SARS-CoV-2-infected cells and organoids. STAR protocols Karim, M., Pohane, A. A., Lo, C. W., Einav, S., Garhyan, J. 2024; 5 (1): 102906

Abstract

Infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research, conducted in high-containment laboratories, requires transferring samples to lower containment labs for downstream applications, mandating sample inactivation. Here, we present a stepwise protocol for chemical inactivation of SARS-CoV-2 virus in culture supernatants or within infected cells and organoids, using eight chemical reagents validated via plaque assays. Additionally, we describe steps for troubleshooting virus inactivation, titer calculation, and log reduction. This protocol offers valuable resources for the COVID-19 research community, providing essential tools to advance research on this virus.

View details for DOI 10.1016/j.xpro.2024.102906

View details for PubMedID 38401122