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Spatially resolved immune exhaustion within the alloreactive microenvironment predicts liver transplant rejection. Science advances Barbetta, A., Rocque, B., Bangerth, S., Street, K., Weaver, C., Chopra, S., Kim, J., Sher, L., Gaudilliere, B., Akbari, O., Kohli, R., Emamaullee, J. 2024; 10 (15): eadm8841

Abstract

Allograft rejection is common following clinical organ transplantation, but defining specific immune subsets mediating alloimmunity has been elusive. Calcineurin inhibitor dose escalation, corticosteroids, and/or lymphocyte depleting antibodies have remained the primary options for treatment of clinical rejection episodes. Here, we developed a highly multiplexed imaging mass cytometry panel to study the immune response in archival biopsies from 79 liver transplant (LT) recipients with either no rejection (NR), acute T cell-mediated rejection (TCMR), or chronic rejection (CR). This approach generated a spatially resolved proteomic atlas of 461,816 cells (42 phenotypes) derived from 96 pathologist-selected regions of interest. Our analysis revealed that regulatory (HLADR+ Treg) and PD1+ T cell phenotypes (CD4+ and CD8+ subsets), combined with variations in M2 macrophage polarization, were a unique signature of active TCMR. These data provide insights into the alloimmune microenvironment in clinical LT, including identification of potential targets for focused immunotherapy during rejection episodes and suggestion of a substantial role for immune exhaustion in TCMR.

View details for DOI 10.1126/sciadv.adm8841

View details for PubMedID 38608023