Sepsis increases NOS-2 activity and decreases non-NOS-mediated acetylcholine-induced dilation in rat aorta 24th Annual Symposium of the Association-of-Veterans-Administration-Surgeons Beach, P. K., Spain, D. A., Kawabe, T., Harris, P. D., Garrison, R. N. ACADEMIC PRESS INC ELSEVIER SCIENCE. 2001: 17–22


Acetylcholine (Ach) is frequently used to assess endothelium-dependent vasodilation during sepsis. However, the effects of sepsis on constitutive nitric oxide synthase activity (NOS-1 and -3) and other non-NOS effects of Ach are unclear.Sepsis was induced in rats by inoculation of an implanted sponge with Escherichia coli and Bacteroides fragilis (10(9) CFU each). Thoracic aortic rings (2 mm) were harvested at 24 h from septic (N = 9) and control (N = 9) rats and were suspended in physiological salt solution (PSS), PSS + l-N(6)-(1-iminoethyl)lysine (l-NIL: NOS-2 inhibitor, 10 microM), or PSS + l-N(G)-monomethylarginine (l-NMMA: NOS-1, -2, and -3 inhibitor, 60 microM). Rings were set at 1-g preload and precontracted with phenlyephrine (10(-8) M). Relaxation dose-response curves were generated with six doses of Ach (3 x 10(-8) to 10(-5) M).Sepsis increased the maximal relaxation to Ach under basal conditions. NOS 2 inhibition with l-NIL decreased Ach-induced relaxation in controls (66% vs 84%, P < 0.05, two-way ANOVA) and more so in septic rats (44% vs 93%, P < 0.05). Total NOS inhibition with l-NMMA decreased Ach-induced relaxation to 45% (P < 0.05) in controls and to 30% (P < 0.05) in septic animals.Inhibition of NOS-1, -2, and -3 failed to abolish Ach-induced relaxation, suggesting the presence of other Ach-induced vasodilator mechanisms. NOS-2 inhibition reduced Ach-induced relaxation by 20-25% in the normal thoracic aorta, but by 50% in septic animals. The remaining Ach-induced non-NOS vasodilation (after inhibition of NOS-1 + NOS-2 + NOS-3) was reduced from 45% in normals to 30% in septic animals. Vascular dysregulation in sepsis is a complex event involving increased NOS-2, decreased NOS-1 + NOS-3, and decreased Ach-induced non-NOS vasodilator mechanisms.

View details for DOI 10.1006/jsre.2000.6056

View details for Web of Science ID 000167252600004

View details for PubMedID 11180991