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Multiplexed protein detection by proximity ligation for cancer biomarker validation NATURE METHODS Fredriksson, S., Dixon, W., Ji, H., Koong, A. C., Mindrinos, M., Davis, R. W. 2007; 4 (4): 327-329

Abstract

We present a proximity ligation-based multiplexed protein detection procedure in which several selected proteins can be detected via unique nucleic-acid identifiers and subsequently quantified by real-time PCR. The assay requires a 1-microl sample, has low-femtomolar sensitivity as well as five-log linear range and allows for modular multiplexing without cross-reactivity. The procedure can use a single polyclonal antibody batch for each target protein, simplifying affinity-reagent creation for new biomarker candidates.

View details for DOI 10.1038/NMETH1020

View details for Web of Science ID 000245584900013

View details for PubMedID 17369836